Figure 2 showcases a simplified version of the sampling process of a female yelloweye rockfish (Sebastes ruberrimus) operculum. First, we clipped each GI (1 light + 1 dark layer, (a, b)). Second, we cut individual GIs into smaller pieces for steroid hormone extraction (c). Finally, we have the end result of sampling all GIs of a yelloweye rockfish operculum (d).
I had three main goals in my dissertation relating to the analysis of hormone deposition in GIs of a female teleost operculum that I wanted to address. First, I wanted to determine if reproductive and stress-related hormones were deposited in the individual GIs of the long-lived (>100 years old!) and human-targeted species, the yelloweye rockfish. Second, I wanted to develop female reproductive parameters (e.g., age of sexual maturity and spawning frequency) from these lifetime hormone profiles to improve models for catch limits. Finally, I wanted to construct models with multiple environmental variables (e.g., sea surface temperature) to determine how environmental changes affect the reproductive (including reproductive parameters) and stress physiology of these fish.
My dissertation research resulted in two publications relating to hormones in yelloweye rockfish opercula, which can be found in my Publications page!